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Developmental expression of two forms of pp60c-src in mouse brain
1Department of Pathology, University of California, San Diego, La Jolla 92093.
Molecular and Cellular Biology
|January 1, 1988
Summary
Mouse brain development reveals distinct expression patterns for protein-tyrosine kinase pp60c-src forms. Initially, only pp60 is present, with pp60+ emerging later and becoming dominant in adult forebrain and midbrain.
Area of Science:
- Developmental Biology
- Molecular Biology
- Neuroscience
Background:
- Protein-tyrosine kinases play crucial roles in cellular signaling pathways.
- The pp60c-src proto-oncogene encodes two related proteins, pp60 and pp60+, with potentially distinct functions.
- Understanding the developmental expression of these isoforms is key to deciphering their roles in brain formation.
Purpose of the Study:
- To investigate the temporal and spatial expression patterns of pp60 and pp60+ during mouse embryonic development.
- To determine the predominant pp60c-src isoform in specific brain regions at different developmental stages.
Main Methods:
- Western blot analysis of whole-brain lysates from mice at various embryonic days (E9, E10, E18).
- Analysis of forebrain and midbrain lysates from adult mice.
Main Results:
- At embryonic day 9 (E9), only the pp60 form of protein-tyrosine kinase was detected in whole-brain lysates.
- A detectable level of pp60+ expression was first observed at embryonic day 10 (E10).
- In E18 embryos and adult mice, pp60+ was the predominant form of pp60c-src in both forebrain and midbrain tissues.
Conclusions:
- The expression of pp60c-src isoforms is developmentally regulated in the mouse brain.
- pp60 is the primary isoform expressed early in development, while pp60+ becomes dominant in later stages and in adult brain regions.
- These findings suggest distinct roles for pp60 and pp60+ in mouse brain development and function.