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Updated: May 3, 2026
Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
Published on: February 3, 2023
Human-derived alternatives to fetal bovine serum in cell culture
Karin Witzeneder1, Andrea Lindenmair2, Christian Gabriel1
1Red Cross Blood Transfusion Service of Upper Austria, Linz, Linz/Vienna, Austria ; Austrian Cluster for Tissue Regeneration, Linz/Vienna, Austria.
Objective:
The need for an alternative to fetal bovine serum (FBS) is known to scientists and users involved in cell therapy or advanced therapy medicinal products. Human serum (huS) and platelet lysate (hPL) can be used as alternatives resulting in similar or even superior results concerning cell expansion.
Methods:
We developed protocols for the production of huS and two types of hPL and tested them in the expansion of human fibroblasts and adipose tissue-derived stem cells (ASC). Quality control included cell counts (platelets, red and white blood cells), sterility testing, pH levels, total protein concentrations and growth factor levels. ASC and fibroblasts were expanded for three passages in media supplemented with FBS, huS or hPL and evaluated microscopically. Proliferation in terms of population doubling times (PDT) was determined. In case of ASC, differentiation was performed as well.
Results:
All three alternatives demonstrated shorter PDT for fibroblasts and ASC compared to FBS. Furthermore, ASC maintained their differentiation potential.
Conclusion:
We conclude that hPL and huS can be used as alternatives to FBS for the cultivation and expansion of cells intended for human use.
