Related Experiment Video
Updated: May 3, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Cane molasses as a source of precursors in the bioproduction of tryptophan by Bacillus subtilis
Marzieh Dehghan Shasaltaneh1, Zahra Moosavi-Nejad1, Sara Gharavi1
1Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran.
Background And Objectives:
The essential amino acid L-tryptophan can be produced by a condensation reaction between indole and L-serine, catalyzed by B. subtilis with tryptophan synthase activity. Application of the tryptophan is widespread in the biotechnology domain and is sometimes added to feed products as a food fortifier.
Materials And Methods:
The optimum concentration of the Iranian cane molasses was determined by measuring the amount of biomass after growth in 1 to 30 g/mL of molasses. The maximum amount of biomass was obtained in 10 g/mL molasses. Chromatographic methods, TLC and HPLC, were used to assay the amount of tryptophan produced in the presence of precursors of tryptophan production (indole and serine) and/or molasses.
Results:
Our results indicate the importance of the Iranian cane molasses not only as carbon source, but also as a source of precursors for tryptophan production.
Conclusion:
This report evaluates the potential of cane molasses as an economical source for tryptophan production by B. subtilis, hence eliminating the requirement for additional serine and indole as precursors.
More Related Videos
08:56Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
09:31PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Published on: September 26, 2020
Related Concept Videos
Production of Pharmaceuticals
Production of Antibiotics
Production of Organic Acids
Amino Acid Biosynthetic Pathways
Microbes in the Production of Fermented Foods
Production of Biopesticides