Advancing Tumor Therapy: Development and Utilization of Protein-Based Nanoparticles

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Materials Science

Background:

• Protein-based nanoparticles (PNPs) are crucial for advanced cancer treatment due to their targeted delivery, low toxicity, and tunable characteristics.
• Effective production methods are essential for utilizing PNPs in diverse biomedical applications like drug delivery, imaging, and tissue engineering.

Purpose of the Study:

• To review and categorize existing and novel methods for producing protein-based nanoparticles (PNPs) and their drug complexes.
• To provide a clear framework for understanding PNP production mechanisms and influencing factors.

Main Methods:

• Classification of PNP production techniques into three categories: solubility change, solvent substitution, and thin flow methods.
• Analysis of underlying principles, influencing factors (size, morphology, stability, functionality), and challenges (scalability, reproducibility, biocompatibility) for each method.

Main Results:

• The proposed classification offers a simplified and advantageous understanding of PNP production compared to other systems.
• Detailed discussion of the advantages and limitations associated with solubility change, solvent substitution, and thin flow methods.

Conclusions:

• Understanding PNP production methods is key to advancing their application in tumor therapy and other biomedical fields.
• Emerging trends and innovative strategies are expected to overcome current limitations, driving future developments in PNP technology.