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Chemical synthesis of r(GCAUGC) for high-resolution 1H-NMR studies
E Happ1, C Scalfi-Happ, G M Clore
1Max-Planck-Institut für Biochemie, Martinsried, FRG.
Nucleic Acids Symposium Series
|January 1, 1987
Abstract:
The chemical synthesis of a short RNA duplex, to be used for 500 MHz 1H-NMR experiments, is presented. The target oligonucleotide was constructed in a blockwise fashion according to the benzotriazolyl phosphotriester approach in solution. The CPTr group, 4,4',4"-tris(4,5-dichlorophthalimido)trityl, was used for temporary protection of the 5'-OH; this blocking group was removed by buffered hydrazine without harming any other protecting group.