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Dithiothreitol: An inhibitor of glucose-6-phosphate-dehydrogenase activity in leaf extracts and isolated chloroplasts
1Department of Botany, University of Queensland, St. Lucia, Queensland, Australia.
Abstract:
The activity of glucose-6-phosphate dehydrogenase (G-6-P-DH; D-glucose 6-phosphate: NADP oxidoreductase, EC 1.1.1.49) in leaf extracts of barley and spinach can be decreased 20-35% by incubation of the leaf extracts with dithiothreitol (DTT). This inhibition is complete within 2 min at 0°C and is reversible. The DTT-inhibited portion of G-6-P-DH activity in leaf extracts is probably that portion of leaf enzyme inhibited during illumination, and evidence has been obtained that this activity is located in the chloroplasts.
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