Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Minicolumn detection methods for aflatoxin in raw peanuts: collaborative study

O L Shotwell, C E Holaday, A K Athnasios

    Journal - Association of Official Analytical Chemists
    |May 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Patients', family members' and healthcare practitioners' experiences of Stevens-Johnson syndrome and toxic epidermal necrolysis: a qualitative descriptive study using emotional touchpoints.

    Journal of the European Academy of Dermatology and Venereology : JEADV·2020
    Same author

    The psychological impact of Stevens-Johnson syndrome and toxic epidermal necrolysis on patients' lives: a Critically Appraised Topic.

    The British journal of dermatology·2019
    Same author

    DNA Polymerase Beta Participates in Mitochondrial DNA Repair.

    Molecular and cellular biology·2017
    Same author

    Dicarboxylic acids as pH sensors for hyperpolarized <sup>13</sup>C magnetic resonance spectroscopic imaging.

    The Analyst·2017
    Same author

    Arachidonic acid: Occurrence in the reproductive tract of the male house cricket (Acheta domesticus) and field cricket (Gryllus spp.).

    Lipids·2016
    Same author

    [(11)C]Ascorbic and [(11)C]dehydroascorbic acid, an endogenous redox pair for sensing reactive oxygen species using positron emission tomography.

    Chemical communications (Cambridge, England)·2016
    Same journal

    GC/MIP/AED method for pesticide residue determination in fruits and vegetables.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Separation and determination of diesel contaminants in various fish products by capillary gas chromatography.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Analysis of organochlorine pesticide residues using simultaneous injection of two capillary columns with electron capture and electrolytic conductivity detectors.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Improved method for determination of polynuclear aromatic hydrocarbons in pharmacopoeial paraffin and mineral oils.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Monitoring aldehyde production during frying by reversed-phase liquid chromatography.

    Journal - Association of Official Analytical Chemists·1991
    Same journal

    Use of the Mycosep multifunctional cleanup column for liquid chromatographic determination of aflatoxins in agricultural products.

    Journal - Association of Official Analytical Chemists·1991
    See all related articles

    The Holaday-Velasco method is superior for detecting aflatoxin in peanuts compared to a modified Holaday method. Holaday-Velasco demonstrated higher accuracy, missing fewer samples and producing no false positives.

    Area of Science:

    • Food Safety
    • Analytical Chemistry
    • Mycotoxin Analysis

    Background:

    • Aflatoxins are toxic compounds produced by molds, posing a significant risk to food safety.
    • Accurate detection methods for aflatoxins are crucial for regulatory compliance and consumer protection.
    • Existing methods like the Holaday method have been refined to improve safety and stability.

    Purpose of the Study:

    • To compare the performance of the Holaday-Velasco method against a modified Holaday method for aflatoxin determination in raw peanuts.
    • To evaluate the accuracy, sensitivity, and specificity of both methods.

    Main Methods:

    • The Holaday-Velasco method, combining Holaday extraction with Velasco's minicolumn, was compared to a modified Holaday method.
    • Modifications to the Holaday method included solvent substitutions (toluene for benzene, methylene chloride for chloroform) and alumina activity adjustment (V to III).

    Related Experiment Videos

  • Both methods were used to analyze raw peanuts with varying levels of aflatoxin contamination, including uncontaminated samples.

    • Main Results:

    • The modified Holaday method missed aflatoxin in 4 out of 42 analyses (13-20 ng/g range), while the Holaday-Velasco method had no misses in this range.
    • Neither method missed aflatoxin in samples with >20 ng/g.
    • The modified Holaday method produced 2 false positives from 14 analyses, whereas the Holaday-Velasco method had no false positives from 15 analyses.

    Conclusions:

    • The Holaday-Velasco method demonstrated superior performance in detecting aflatoxin in raw peanuts, with higher accuracy and fewer false positives.
    • The modifications made to the Holaday method did not fully compensate for its limitations compared to the Holaday-Velasco approach.
    • The Holaday-Velasco method was officially adopted for peanut analysis, highlighting its reliability.