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Updated: May 3, 2026

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Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
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Embryoids derived from isolated protoplasts of carrot
1Institute for Agricultural Research, Tohoku University, Sendai, Japan.
Planta
|February 1, 2014
Abstract:
Protoplasts isolated enzymatically from carrot root tissues developed into cell clusters in a liquid medium containing coconut milk and naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Cells of the resulting calluses differentiated into embryoids on an agar medium containing coconut milk or kinetin.
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