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Updated: May 3, 2026
17:38
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
Published on: April 27, 2012
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Radioactive in situ hybridization to detect gene expression in skeletal tissue sections
Kyu Sang Joeng1, Jenna Regan2, Fanxin Long2
1Baylor College of Medicine, Houston, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2014
Abstract:
In situ hybridization (ISH) using RNA probes is a valuable technique to characterize gene expression patterns in animal tissues. It provides valuable spatial information about gene expression. Compared to the nonradioactive alternatives,(35)S radioactive ISH generally provides higher sensitivity. Here, we describe the procedure for(35)S ISH on paraffin sections from the skeletal tissues of mouse embryos.
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