Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A developmentally regulated antigen associated with neural cell and process migration.

R Mendez-Otero1, B Schlosshauer, C J Barnstable

  • 1Department of Biology, Yale University, New Haven, Connecticut 06511.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|February 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biological behavior of mesenchymal stem cells on poly-ε-caprolactone filaments and a strategy for tissue engineering of segments of the peripheral nerves.

Stem cell research & therapy·2015
Same author

Monoclonal antibodies - tools to dissect the nervous system.

Immunology today·2014
Same author

Prostate carcinoma cell growth-inhibiting hydrogel supports axonal regeneration in vitro.

Neuroscience letters·2013
Same author

Bioactive coronary stent coating based on layer-by-layer technology for siRNA release.

Acta biomaterialia·2013
Same author

The recombinant bifunctional protein αCD133-GPVI promotes repair of the infarcted myocardium in mice.

Journal of thrombosis and haemostasis : JTH·2012
Same author

Role of the 9-O-acetyl GD3 in subventricular zone neuroblast migration.

Molecular and cellular neurosciences·2011

The JONES antibody recognizes an epitope associated with neural cell migration and axon growth during rat nervous system development. This binding is prominent in fetal and early postnatal stages, becoming restricted to the retina and cerebellum in adults.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Immunohistochemistry

Background:

  • Understanding the molecular mechanisms guiding neural cell migration and axon growth is crucial for comprehending nervous system development.
  • The monoclonal antibody JONES recognizes a specific epitope whose distribution in the developing nervous system has not been fully characterized.

Purpose of the Study:

  • To investigate the distribution of the JONES epitope in the developing rat nervous system.
  • To test the hypothesis that JONES binding is associated with neural cell migration and axon growth.

Main Methods:

  • Immunohistochemistry using indirect immunofluorescence and immunogold procedures.
  • Analysis of rat nervous system tissues from embryonic day 9 to adulthood.
  • Focus on well-characterized regions of cell and axon migration.

Related Experiment Videos

Main Results:

  • JONES immunoreactivity first appears at embryonic day 11-12 in specific neural tube regions.
  • Intense labeling observed in the ventricular zone with radial patterns in intermediate and marginal zones during embryonic and early postnatal life.
  • Expression correlates with cell migration (retina, superior colliculus, cerebellum, telencephalon) and neurite extension (optic tract, cerebellum white matter, dorsal roots, trigeminal system, olfactory nerve).
  • Labeling becomes restricted postnatally, with adult expression limited to the retina and cerebellum; also noted in adrenal medulla and kidney glomeruli.

Conclusions:

  • The antigens recognized by the JONES antibody are associated with the migration of specific cell populations and axons in the developing rat nervous system.
  • These antigens may play a role in mediating selectivity during neural cell migration and axon guidance.