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Updated: May 3, 2026
Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
Published on: October 9, 2021
Temperature-sensitive electrochemical recognition of tryptophan enantiomers based on β-cyclodextrin self-assembled on
Yongxin Tao1, Jiangying Dai, Yong Kong
1Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University , Changzhou 213164, P. R. China.
Abstract:
A poly(l-glutamic acid)/β-cyclodextrin (P-l-Glu/β-CD)-modified glassy carbon electrode (GCE) was prepared by electrochemical polymerization of l-glutamic acid on GCE and subsequent self-assembly of β-CD onto the obtained P-l-Glu. Electrochemical recognition of tryptophan (Trp) enantiomers with the P-l-Glu/β-CD was investigated by differential pulse voltammetry (DPV), and the results show that Trp enantiomers can be effectively recognized at the P-l-Glu/β-CD-modified GCE. It is also interesting to find that temperature plays a crucial role in the enantioselective recognition, which is evidenced by the variable-temperature UV spectra of the inclusion complexes of Trp enantiomers and β-CD. Under optimum conditions, the oxidation peak current ratio of l-Trp to d-Trp could reach 2.30, which is attributed to the stereoselectivity of β-CD to the enantiomeric pair of Trp.
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