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Updated: May 3, 2026

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique
Published on: November 11, 2022
Crebanine inhibits voltage-dependent Na+ current in guinea-pig ventricular myocytes
He Xiao-Shan1, Lin Qing1, Ma Yun-Shu1
1Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
Aim:
To study the effects of crebanine on voltage-gated Na(+) channels in cardiac tissues.
Methods:
Single ventricular myocytes were enzymatically dissociated from adult guinea-pig heart. Voltage-dependent Na(+) current was recorded using the whole cell voltage-clamp technique.
Results:
Crebanine reversibly inhibited Na(+) current with an IC50 value of 0.283 mmol·L(-1) (95% confidence range: 0.248-0.318 mmol·L(-1)). Crebanine at 0.262 mmol·L(-1) caused a negative shift (about 12 mV) in the voltage-dependence of steady-state inactivation of Na(+) current, and retarded its recovery from inactivation, but did not affect its activation curve.
Conclusion:
In addition to blocking other voltage-gated ion channels, crebanine blocked Na(+) channels in guinea-pig ventricular myocytes. Crebanine acted as an inactivation stabilizer of Na(+) channels in cardiac tissues.
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