Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Applications Of NMR In Biology

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.

Two-Dimensional (2D) NMR: Overview

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....

2D NMR: Overview of Homonuclear Correlation Techniques

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

2D NMR: Overview of Heteronuclear Correlation Techniques

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...

¹H NMR: Complex Splitting

¹H NMR: Complex Splitting

A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural Significance and Reference Value of High-Precision Coupling Constants and Raw Data Sharing.

Analytical chemistry·2026

Same author

There is No Caffeine in Damiana-Dismantling Filter Bubbles and Echo Chambers in Scientific Referencing.

Planta medica·2026

Same author

Multilevel Residual Complexity Analysis Reveals Origin of Nanomolar Antiviral Bioactives of "Isoquercitrin".

Journal of natural products·2026

Same author

Long-term nano-dynamic mechanical analysis and micro-permeability of adhesive interface components using four AB-type proanthocyanidin neutral primers.

Journal of dentistry·2026

Same author

NMR-Based Quantification of Collagen Content in Protein Hydrolysates.

Journal of agricultural and food chemistry·2026

Same author

From benzyl to fluorenyl: enhancing photostability and modulating optoelectronic properties of <i>para</i>-azaquinodimethanes.

Chemical communications (Cambridge, England)·2026

Search research articles

Related Experiment Video

Updated: May 3, 2026

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Published on: April 7, 2012

Universal quantitative NMR analysis of complex natural samples.

Charlotte Simmler¹, José G Napolitano¹, James B McAlpine¹

¹UIC/NIH Center for Botanical Dietary Supplements Research, Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, College of Pharmacy, 833 S. Wood Street, Chicago, IL, United States.

Current Opinion in Biotechnology

|February 4, 2014

Summary

Nuclear Magnetic Resonance (NMR) is a quantitative technique used for identifying and quantifying drug metabolites. Quantitative NMR (qNMR) is increasingly vital for analyzing complex samples like foods and biofluids in pharmaceutical research and clinical diagnosis.

More Related Videos

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

Related Experiment Videos

Last Updated: May 3, 2026

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Published on: April 7, 2012

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts

Published on: September 21, 2014

Area of Science:

Analytical Chemistry
Biochemistry
Pharmaceutical Sciences

Background:

Nuclear Magnetic Resonance (NMR) is a universal and quantitative analytical technique.
NMR serves as a unique structural tool and competes with metrological techniques for purity determination and reference material analysis.

Purpose of the Study:

To highlight the applications of quantitative NMR (qNMR) in pharmaceutical research, focusing on drug and biological metabolite identification and quantification.
To emphasize the growing importance of NMR-based metabolomic studies for analyzing complex natural samples and biofluids in quality control and clinical diagnosis.

Main Methods:

Utilizing the quantitative nature of NMR spectroscopy for precise compound identification and measurement.
Applying NMR for simultaneous quantification of multiple compounds within complex sample matrices.
Leveraging NMR for unbiased sample composition analysis in metabolomic studies.

Main Results:

qNMR enables accurate identification and quantification of drug and biological metabolites in pharmaceutical research.
qNMR provides an unbiased view of sample composition, allowing for simultaneous quantification of multiple analytes.
NMR-based metabolomic studies are increasingly adopted for characterizing complex natural products and biofluids.

Conclusions:

qNMR is a powerful method for metabolomic studies and quality control of diverse samples, including foods, plants, herbal remedies, and biofluids.
The application of NMR in metabolomics for herbal remedy characterization and clinical diagnosis has seen significant growth.