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The pyruvate dehydrogenase complex of Corynebacterium glutamicum: an attractive target for metabolic engineering
Bernhard J Eikmanns1, Bastian Blombach2
1Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
Abstract:
The pyruvate dehydrogenase complex (PDHC) catalyzes the oxidative thiamine pyrophosphate-dependent decarboxylation of pyruvate to acetyl-CoA and CO2. Since pyruvate is a key metabolite of the central metabolism and also the precursor for several relevant biotechnological products, metabolic engineering of this multienzyme complex is a promising strategy to improve microbial production processes. This review summarizes the current knowledge and achievements on metabolic engineering approaches to tailor the PDHC of Corynebacterium glutamicum for the bio-based production of l-valine, 2-ketosiovalerate, pyruvate, succinate and isobutanol and to improve l-lysine production.
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