[Studies on the liberation behavior of insulin from polymeric matrices]

The in-vitro and in-vivo liberation of insulin from embeddings in polymeric matrices was investigated dependent on the charging degree, the particle- and pore size, respectively, of the granules and the dissolution behaviour of the hormone. Polyacrylamide, agar-agar, dextrane and Sephadex G-100 were used as ground-mass. These polymers are characterized by a good swelling and a high binding capacity. If these properties quality them for insulin was investigated in model experiments. Information concerning the obtained retention effects of the matrix mould were produced by photochemical methods (application of insulin against citrate phosphate buffer; absorption region of 230 to 320 nm). The in-vitro effects yielded could be confirmed by means of representative in-vivo studies in eumetabolic insulin sensitive rabbits.