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Updated: May 3, 2026

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
Published on: September 12, 2025
Recent advances in in vivo applications of intein-mediated protein splicing
Natalya I Topilina, Kenneth V Mills1
1Department of Chemistry, College of the Holy Cross, 1 College Street, Worcester, MA 01610, USA. kmills@holycross.edu.
Abstract:
Intein-mediated protein splicing has become an essential tool in modern biotechnology. Fundamental progress in the structure and catalytic strategies of cis- and trans-splicing inteins has led to the development of modified inteins that promote efficient protein purification, ligation, modification and cyclization. Recent work has extended these in vitro applications to the cell or to whole organisms. We review recent advances in intein-mediated protein expression and modification, post-translational processing and labeling, protein regulation by conditional protein splicing, biosensors, and expression of trans-genes.
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