Effect of hyperoxic exposure on protein synthesis in the rat

Rates of protein synthesis were measured in vivo [corrected] in the lung and heart from fed rats exposed to hyperoxia (less than or equal to 95% O2) for either 6 or 24 h. Protein synthesis rates were depressed by 16-32% compared with normoxic controls in these tissues. The inhibition in both tissues was greatest after 24 h hyperoxic exposure. The decreased fractional rates of synthesis in both tissues were related to changes in ribosomal activity rather than capacity. The fall in synthesis rate per ribosome was greatest in both tissues when the exposure period was increased to 24 h. The possible mechanism(s) involved in hyperoxia-induced depression of protein synthesis are discussed.