Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Sarcoplasmatic reticulum function in the ischemic myocardium.

J Blom¹, P D Verdouw, J M Lamers

¹Department of Biochemistry, Erasmus University Rotterdam, The Netherlands.

Biomedica Biochimica Acta

|January 1, 1987

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The introduction of a new mobile lifestyle tool in the battle against hypothalamic obesity; the "HAPPYthalamus" application.

Obesity pillars·2025

Same author

Inflammatory bowel disease and risk of adenocarcinoma and neuroendocrine tumors in the small bowel.

Annals of oncology : official journal of the European Society for Medical Oncology·2022

Same author

Emergency upscaling of video consultation during the COVID-19 pandemic: Contrasting user experience with data insights from the electronic health record in a large academic hospital.

International journal of medical informatics·2021

Same author

Codon Usage Heterogeneity in the Multipartite Prokaryote Genome: Selection-Based Coding Bias Associated with Gene Location, Expression Level, and Ancestry.

mBio·2019

Same author

Genomic analysis of a novel nontoxigenic <i>Corynebacterium diphtheriae</i> strain isolated from a cancer patient.

New microbes and new infections·2019

Same author

Circulating serum miR-223-3p and miR-16-5p as possible biomarkers of early rheumatoid arthritis.

Clinical and experimental immunology·2018

Same journal

Intracerebroventricular administration of hypertonic sodium chloride solution reduces the sensitivity of the baroreceptor heart reflex in anaesthetized rats.

Biomedica biochimica acta·1991

Same journal

An efficient chemical-enzymatic synthesis of LHRH N-terminal pentapeptide.

Biomedica biochimica acta·1991

Same journal

Adrenergic cardiovascular actions in rats as affected by dichloromethane exposure.

Biomedica biochimica acta·1991

Same journal

Lipoxygenase-inhibitory action of antiviral polymeric oxidation products of polyphenols.

Biomedica biochimica acta·1991

Same journal

Uptake of lysine and incorporation into proteins of the cortex during a short-term hypoxia of neonatal rats.

Biomedica biochimica acta·1991

Same journal

Influence of intestinal resection, type of dietary fat and time on the digestive and metabolic utilization of fat in rats.

Biomedica biochimica acta·1991

See all related articles

Myocardial ischemia impairs sarcoplasmic reticulum function, reducing calcium pumping ATPase activity. This damage, evident after just 0.5 hours, may stem from altered second messenger control of the calcium pump.

Area of Science:

Cardiology
Cellular Biology
Biochemistry

Background:

Myocardial ischemia, a critical condition in cardiovascular disease, significantly impacts heart muscle function.
The sarcoplasmic reticulum plays a vital role in regulating intracellular calcium levels, essential for cardiac contraction.
Understanding the effects of ischemia on sarcoplasmic reticulum function is crucial for developing therapeutic strategies.

Purpose of the Study:

To investigate the impact of varying durations of myocardial ischemia on sarcoplasmic reticulum function.
To assess the effects of ischemia on the Ca2+ pumping ATPase activity and phospholamban phosphorylation in porcine hearts.

Main Methods:

Porcine hearts were subjected to successive occlusions of the left anterior descending coronary artery to induce myocardial ischemia for 0.5, 1, or 2 hours.

Related Experiment Videos

Sarcoplasmic reticulum vesicles were isolated from transmural biopsies of both control and ischemic myocardial segments.

Ca2+ pumping ATPase activity and in vitro phosphorylation of phospholamban were measured.

Main Results:

Ca2+ pumping ATPase activity was significantly impaired after 0.5 hours of ischemia (77% of control) and further reduced after 1 hour (44% of control).
The functional damage to the sarcoplasmic reticulum persisted with longer ischemic periods.
In vitro phosphorylation of phospholamban by the catalytic subunit was also reduced, suggesting altered second messenger control.

Conclusions:

Myocardial ischemia rapidly impairs sarcoplasmic reticulum Ca2+ pumping ATPase function.
The observed functional damage may be linked to alterations in the second messenger system regulating the Ca2+ pump.
These findings highlight the vulnerability of sarcoplasmic reticulum function to ischemic insult and its potential contribution to cardiac dysfunction.