Related Experiment Video
Updated: May 3, 2026

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels
Published on: August 7, 2017
Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic
Gwo-Hwa Wan1, Dah-Chin Yan2, Hui-Yun Tseng3
1Department of Respiratory Therapy, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Abstract:
This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19 ng/μL, and the average LTB4 concentration of each EBC sample was 15.38 ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.
More Related Videos
13:45Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
Published on: December 23, 2010
07:32Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019