Methods for retrovirus-mediated gene transfer into primary T-lymphocytes

Recombinant retroviruses are efficient vectors for introducing genes into many mammalian cell types. They are useful in the context of clinical as well as experimental applications, owing to their ability to generate high-titer and helper-free viral stocks. Retroviral vectors are especially appropriate for the transduction of primary lymphocytes, because gene transfer is mediated by nonimmunogenic vectors and stable vector integration in the target genome is achieved. Stable integration in cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells' progeny.