Related Experiment Video
Updated: May 3, 2026
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Detection of Caprine-specific Nucleic Acid Sequences in Goat Milk Using Polymerase Chain Reaction
Asim A Osman1, Imadeldin E Aradaib2, Omer A Musa3
1Molecular Biology Laboratory, Department of Clinical Medicine, Faculty of Veterinary Medicine, University Khartoum , Sudan ; Department of Physiology, Faculty of Medicine, Gadarif University , Gadarif, Sudan.
Conflict Of Interest:
none declared.
Introduction:
This study was carried out to evaluate PCR-based method for detection of DNA in goat milk. It utilized primers targeting the mitochondrial cytochrome -b (mtcyt-b) gene, which was used as a target DNA for PCR amplification.
Methods:
For the specific identification of goat mtcyt-b gene, pair of primers (GSL1, GSR2), were used, which produced a 428 base pair (bp) PCR product from milk samples as well as from peripheral blood. Amplification products were visualized on ethidium bromide-stained agarose gels.
Results And Discussion:
Amplification products were not detected when the PCR was applied to DNA from animal species including cattle, sheep, swine, camel, deer, horse, donkey, and human, which indicates that the 2 pairs of primers are specific for goat.
Conclusion:
DNA can be extracted from goat milk and would be advantageous in the variety of application such as species identification in milk and milk.
Related Concept Videos
01:32PCR - Polymerase Chain Reaction
02:57Real Time RT-PCR
The real-time quantification of the number of amplified products is...