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Indole-based alkaloids from deep-sea bacterium Shewanella piezotolerans with antitumor activities
Yao Wang1, Xixiang Tang2, Zhongzhe Shao2
1State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, PR China.
Abstract:
Chromatographic separation of a crude extract obtained from a fermentation broth of a chemically unknown bacterium Shewanella piezotolerans WP3 collected in deep-sea yielded three new indole alkaloids namely shewanellines A (1a), B (1b) and C (2), together with 12 known indole alkaloids. The structures were unambiguously elucidated on the basis of 1D and 2D NMR ((1)H, (13)C, COSY, HMBC, HSQC and NOESY) in association with MS and CD data. Compounds 1-4, 7, 9 and 11-14 were selected for the evaluation of their cytotoxic activities against human tumor cell lines HL-60 and BEL-7402, whereas compounds 2, 4 and 9 exhibited significant inhibition toward HL-60.
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