Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tissue-Drug Binding: Localization of Drugs and its Significance

Tissue-Drug Binding: Localization of Drugs and its Significance

Body tissues, comprising approximately 40% of the body weight, are crucial in drug distribution and localization. These tissues can serve as drug storage sites, competing with plasma binding sites for drug molecules.
Drugs can bind to different tissue components, enhancing their distribution and localization. The factors influencing drug localization in tissues include the drug's lipophilicity, structural characteristics, tissue perfusion rate, and pH differences. These factors determine...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clarification and confocal imaging of the nonhuman primate placental micro-anatomy.

BioTechniques·2018

Same author

Growth and regeneration of waxes on the leaves of Eucalyptus.

Planta·2014

Same author

Embryogenesis and germination in rye (Secale cereale L.) : 1. Fine structure of the developing embryo.

Planta·2014

Same author

Embryogenesis and germination in rye (Secale cereale L.) : II. Biochemical and fine structural changes during germination.

Planta·2014

Same author

Orientation of cell growth in the etiolated pea stem : Effect of ethylene and auxin on cell wall deposition.

Planta·2014

Same author

Embryogenesis and germination in rye (Secale cereale L.) : III. Fine structure and biochemistry of the non-viable embryo.

Planta·2014

Search research articles

Related Experiment Video

Updated: May 3, 2026

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

The localization of 2,4-D in leaf tissue.

N D Hallam¹, J A Sargent

¹A.R.C. Unit of Experimental Agronomy, Department of Agricultural Science, University of Oxford, England.

|February 6, 2014

Summary

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) disrupts chloroplast structure in Phaseolus vulgaris. This compound is primarily found in chloroplasts extracted using organic solvents from freeze-dried tissue.

Area of Science:

Plant biology
Herbicide research
Cellular biology

Background:

2,4-dichlorophenoxyacetic acid (2,4-D) is a widely used herbicide.
Application of 2,4-D to Phaseolus vulgaris leaves causes disruption of chloroplast structure.

Purpose of the Study:

To investigate the localization of 2,4-D and its metabolites within Phaseolus vulgaris cells after herbicide application.
To determine the primary cellular components retaining the herbicide.

Main Methods:

Extraction of chloroplasts from Phaseolus vulgaris leaves treated with 2,4-D using aqueous and organic solvent mediums.
Analysis of herbicide content in extracted chloroplasts.

Main Results:

Chloroplasts extracted in an aqueous medium were free of 2,4-D and its metabolites.

More Related Videos

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

Published on: September 7, 2015

Related Experiment Videos

Last Updated: May 3, 2026

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay

Published on: September 7, 2015

Chloroplasts extracted from freeze-dried tissue using organic solvents were the main cellular components containing the herbicide.

Conclusions:

The method of chloroplast extraction significantly influences the detection of 2,4-D.
Chloroplasts are a primary site of 2,4-D accumulation in Phaseolus vulgaris, particularly when extracted with organic solvents.