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Updated: May 3, 2026
Author Spotlight: Optimizing Antibody-Based Cancer Treatments via Antibody-Dependent, Cell-Mediated Cytotoxicity Assay
Published on: September 13, 2024
Methods for measuring antibody-dependent cell-mediated cytotoxicity in vitro
Aaron S Miller1, Max L Tejada, Hélène Gazzano-Santoro
1Analytical Development and Quality Control, Genentech Inc., South San Francisco, CA, USA.
Abstract:
Antibody-dependent cell-mediated cytotoxicity (ADCC) is a relevant characteristic to measure for a number of therapeutic monoclonal antibodies (mAbs) under development. ADCC is a mechanism by which antibody-opsonized, infected, or cancerous cells are destroyed by FcγRIII (CD16)-expressing effector cells. Here we describe three methods that can be used to quantify the ADCC activity of mAbs by measuring distinct aspects of the ADCC mechanism.
