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Muscle biopsy samples for histochemical processing: alterations induced by storage
1Neuromuscular Laboratory of the Scott-Ritchey Research Program, College of Veterinary Medicine, Auburn University, AL.
Abstract:
Skeletal muscle samples from two healthy dogs were stored in ice at 0 C for up to 30 hours to examine the influence of time on cell morphology and morphometry. Cytochemical and histochemical properties of muscle to 18 hours were not markedly different from fresh frozen tissue. Samples stored to 30 hours were still satisfactory, despite a decline and unevenness in depth of staining. Morphometry from samples stored at 0 C for 6 hours or longer is not recommended, due to the statistically significant increase in diameter (from 21 to 25%) of type I and type II fibers.
Insights
Canine skeletal muscle stored on ice for up to 30 hours shows minimal changes in tissue properties. However, morphometric analysis is unreliable after 6 hours due to significant fiber diameter increases.
Area of Science:
- Veterinary Pathology
- Muscle Biology
- Histology
Background:
- Proper preservation of skeletal muscle tissue is crucial for accurate morphological and morphometric analysis in veterinary diagnostics.
- Understanding the impact of cold storage duration on canine muscle tissue is essential for reliable research and clinical applications.
Purpose of the Study:
- To investigate the effects of cold storage at 0°C on the morphology and morphometry of canine skeletal muscle over a 30-hour period.
- To determine the optimal storage time for maintaining tissue integrity for cytochemical, histochemical, and morphometric evaluations.
Main Methods:
- Skeletal muscle samples from two healthy dogs were collected and stored in ice at 0°C.
- Samples were analyzed at various time points up to 30 hours for cytochemical and histochemical properties.
- Morphometric analysis was performed to assess fiber diameter changes.
Main Results:
- Cytochemical and histochemical properties remained largely unchanged up to 18 hours of cold storage.
- Tissue stored for up to 30 hours was considered satisfactory, though staining intensity showed some decline and unevenness.
- A statistically significant increase (21-25%) in type I and type II fiber diameter was observed in samples stored for 6 hours or longer.
Conclusions:
- Canine skeletal muscle can be stored at 0°C for up to 18-30 hours for general histological assessment with acceptable results.
- Morphometric analysis of canine skeletal muscle is compromised after 6 hours of cold storage at 0°C due to significant fiber swelling.