Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the Blue Zones: Healthy Aging and Extreme Longevity in Italy (1982-2025)-An Ecological Analysis of Demographic, Metabolic, and Nutritional Correlates.

Nutrients·2026
Same author

Editorial: Advanced therapies for cardiac regeneration, volume II.

Frontiers in bioengineering and biotechnology·2026
Same author

Boronate-crosslinked hyaluronic acid hydrogels with grafting-dependent mechano-redox properties.

Biomaterials advances·2026
Same author

Rational design of microfluidic templated HA-LPEI nanogels for the targeted delivery of doxorubicin.

Journal of materials chemistry. B·2026
Same author

Bioinspired scaffold design using a custom Voronoi path generator for extrusion-based 3D printing.

Biomaterials science·2026
Same author

Editorial for the ESB 2025 collection.

Biomaterials science·2026
Same journal

Equity considerations in COVID-19 vaccine allocation modelling: a methodological study.

Interface focus·2025
Same journal

Ethical considerations in infectious disease modelling for public health policy: the case of school closures.

Interface focus·2025
Same journal

Why population heterogeneity matters for modelling infectious diseases.

Interface focus·2025
Same journal

Improving modelling for epidemic response: a progress update from a community of UK infectious disease modellers.

Interface focus·2025
Same journal

Optimization of school closures during an Omicron epidemic in Hong Kong: a modelling study.

Interface focus·2025
Same journal

Impact of opinion dynamics on recurrent pandemic waves: balancing risk aversion and peer pressure.

Interface focus·2025
See all related articles

Related Experiment Video

Updated: May 3, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

14.2K

Polyurethane-based scaffolds for myocardial tissue engineering.

Valeria Chiono1, Pamela Mozetic2, Monica Boffito1

  • 1Department of Mechanical and Aerospace Engineering , Politecnico di Torino , Corso Duca degli Abruzzi 24, Turin , Italy.

Interface Focus
|February 7, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed poly(ester urethane) scaffolds using melt-extrusion additive manufacturing for potential myocardial regeneration. These elastomeric scaffolds supported cardiac progenitor cell adhesion but did not enhance proliferation, indicating future functionalization is needed.

Keywords:
additive manufacturingcardiac progenitor cellsmyocardial tissue engineeringpolyurethane

More Related Videos

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

1.2K
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

8.3K

Related Experiment Videos

Last Updated: May 3, 2026

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

14.2K
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

1.2K
A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

8.3K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Myocardial regeneration requires advanced scaffolds that mimic native tissue properties.
  • Poly(ester urethane)s (PUs) offer tunable mechanical properties suitable for cardiac applications.
  • Additive manufacturing enables precise scaffold fabrication for complex tissue engineering.

Purpose of the Study:

  • To synthesize and fabricate bi-layered poly(ester urethane) scaffolds using melt-extrusion additive manufacturing.
  • To evaluate the scaffold's structural fidelity, mechanical properties, and biocompatibility with human cardiac progenitor cells (CPCs).
  • To assess the potential of these scaffolds for myocardial regeneration applications.

Main Methods:

  • Poly(ester urethane) synthesized from poly(ε-caprolactone) diol, 1,4-butandiisocyanate, and l-lysine ethyl ester dihydrochloride.
  • Melt-extrusion additive manufacturing used to create 0°/90° bi-layered scaffolds.
  • Rheological analysis, differential scanning calorimetry, and thermogravimetry used to determine optimal processing temperature (155°C).
  • In vitro cell culture studies with human cardiac progenitor cells (CPCs) to assess adhesion, spreading, and proliferation.

Main Results:

  • Scaffolds accurately reproduced computer-aided design geometry with an elastomeric-like behavior.
  • Poly(ester urethane) scaffolds demonstrated good biocompatibility, supporting human cardiac progenitor cell adhesion and spreading.
  • No significant stimulation of CPC proliferation was observed over 1-14 days of culture.

Conclusions:

  • Melt-extrusion additive manufacturing is a viable technique for producing poly(ester urethane) scaffolds with excellent structural fidelity and elastomeric properties.
  • The fabricated scaffolds support initial cardiac progenitor cell attachment, a crucial step for tissue regeneration.
  • Surface functionalization of scaffolds with bioactive molecules is recommended to promote CPC proliferation and enhance myocardial regeneration potential.