Virtual tool mark generation for efficient striation analysis
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Updated: May 3, 2026

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
Published on: October 15, 2014
Laura Ekstrand1, Song Zhang, Taylor Grieve
1Ames Laboratory, Iowa State University, 2096 Black Engineering, Ames, IA, 50011.
Abstract:
This study introduces a tool mark analysis approach based upon 3D scans of screwdriver tip and marked plate surfaces at the micrometer scale from an optical microscope. An open-source 3D graphics software package is utilized to simulate the marking process as the projection of the tip's geometry in the direction of tool travel. The edge of this projection becomes a virtual tool mark that is compared to cross-sections of the marked plate geometry using the statistical likelihood algorithm introduced by Chumbley et al. In a study with both sides of six screwdriver tips and 34 corresponding marks, the method distinguished known matches from known nonmatches with zero false-positive matches and two false-negative matches. For matches, it could predict the correct marking angle within ±5-10°. Individual comparisons could be made in seconds on a desktop computer, suggesting that the method could save time for examiners.
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