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Related Experiment Video

Updated: May 3, 2026

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

Microinjection methods for neutrophils.

Iraj Laffafian¹, Kimberly J Lewis, K Benjamin Masterman

¹Neutrophil Signalling Group, School of Medicine, Cardiff University, Cardiff, UK.

Methods in Molecular Biology (Clifton, N.J.)

|February 8, 2014

Summary

This summary is machine-generated.

Microinjecting substances into living neutrophils is now possible using novel methods. Simple lipid-assisted microinjection (SLAM) and electroinjection overcome limitations of conventional techniques for cell manipulation.

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Last Updated: May 3, 2026

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Published on: May 10, 2022

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Published on: May 12, 2018

Area of Science:

Cell biology
Immunology
Biotechnology

Background:

Neutrophils are crucial immune cells, but their manipulation is challenging.
Conventional microinjection methods are ineffective for neutrophils.
Intracellular manipulation allows for studying cell chemistry and function.

Purpose of the Study:

To develop effective microinjection techniques for living neutrophils.
To enable intracellular delivery of substances and indicators into neutrophils.
To facilitate research on neutrophil function and signaling pathways.

Main Methods:

Simple Lipid-Assisted Microinjection (SLAM): A novel technique utilizing lipids for enhanced cell entry.
Electroporation-based microinjection: Employing electrical pulses to facilitate substance delivery.
Both methods were optimized for application to living neutrophils.

Main Results:

Successful microinjection of substances into the neutrophil cytosol was achieved using both SLAM and electroinjection.
These techniques overcome the limitations of traditional glass pipette microinjection for neutrophils.
The developed methods allow for the introduction of otherwise inaccessible molecules and indicators.

Conclusions:

SLAM and electroinjection provide viable methods for microinjecting living neutrophils.
These techniques expand the possibilities for manipulating neutrophil intracellular chemistry and monitoring cellular processes.
This advancement supports further research into neutrophil biology and immune responses.