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Related Concept Videos

Cell Migration01:09

Cell Migration

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Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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Cell Migration01:19

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Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
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Chemotaxis and Direction of Cell Migration01:21

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Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
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Cancer Cell Migration through Invadopodia01:35

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Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
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Role of Myosin in Cell Migration01:18

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Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
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Cytoskeletal Coordination in Cell Migration01:32

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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration
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Neutrophil migration through extracellular matrix.

Richard T Jennings1, Ulla G Knaus

  • 1Conway Institute, University College Dublin, Dublin, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2014
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Summary
This summary is machine-generated.

Neutrophil migration assays help understand immune responses. This study uses a 3D collagen matrix to quantitatively assess neutrophil movement towards chemoattractants, providing insights into immune cell recruitment.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Neutrophil migration is crucial for host defense against infection and injury.
  • Neutrophil locomotion involves diverse modes, including mesenchymal and amoeboid movement, shape changes, and matrix traversal.
  • Existing in vitro assays partially capture the complexity of in vivo neutrophil recruitment.

Purpose of the Study:

  • To establish and utilize a 3D in vitro migration assay for quantitative assessment of neutrophil recruitment.
  • To evaluate the ability of neutrophils to migrate through a collagen type I matrix towards a chemoattractant gradient.

Main Methods:

  • Utilized a commercially available setup for 3D neutrophil migration.
  • Employed a loose collagen type I matrix to mimic tissue structures.
  • Quantitatively assessed migrated neutrophils over a defined time period using a transwell system.

Main Results:

  • The assay enabled quantitative assessment of neutrophil migration through a 3D matrix.
  • The method allowed evaluation of neutrophil attachment, polarization, matrix digestion, and movement.
  • This setup provides a robust system for studying neutrophil recruitment dynamics.

Conclusions:

  • The developed 3D in vitro assay effectively quantifies neutrophil migration through a collagen matrix.
  • This method offers a valuable tool for studying immune cell recruitment in various biological contexts.
  • Further studies can explore neutrophil migration modes within the matrix using this system.