Related Experiment Video
Updated: Jan 10, 2026
Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
Published on: October 14, 2021
High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis
Flavia S Lakschevitz1, Michael Glogauer
1Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada.
Abstract:
The oral cavity is a source of readily available neutrophils and can be used as a model to better understand the role of neutrophils in chronic inflammatory diseases such as rheumatoid arthritis, bronchitis, periodontitis, and inflammatory bowel disease. In this chapter we describe reproducible methods to obtain highly purified neutrophil samples from blood and saliva in humans to enable cell analysis using whole-genome microarrays.
