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Contributions to the phytochemical study of Bidens tripartitae herba from Romania. I. Tannins
M Zagnat1, Corina Cheptea1, A F Spac2
1Faculty of Medical Bioengineering, University of Medicine and Pharmacy Grigore T. Popa, Iasi.
Aim:
To analyze qualitatively and quantitatively tannins in the native plant, collected during the whole vegetation period from different areas of the country, and in its different organs (flower, stem, leaf).
Material And Methods:
For quantitative analysis, the plant product was extracted by repeated maceration (3 days) with 80% methanol. Proanthocyanidins in the extract were quantified by spectrophotometric methods.
Results:
condensed tannins were present while hydrolyzed tannins were absent. Chromatographic analysis showed that tannins spectrum is similar in all plant organs and in plants collected at different times throughout the vegetation period. The differences are only quantitative. The maximum amount of tannins was found during the flowering stage (10.32%).
Conclusions:
In terms of tannin content, flowering is the best time to collect. However, collection throughout the whole vegetation period is acceptable.
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