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In situ forming spruce xylan-based hydrogel for cell immobilization
Volodymyr Kuzmenko1, Daniel Hägg2, Guillermo Toriz3
1Wallenberg Wood Science Center, Kemivägen 4, SE-412 96 Gothenburg, Sweden; Department of Microtechnology and Nanoscience, Chalmers University of Technology, Kemivägen 9, SE-412 96 Gothenburg, Sweden.
Carbohydrate Polymers
|February 11, 2014
Summary
Spruce xylan forms an in situ hydrogel for cell delivery. This novel biomaterial supports mesenchymal stem cell differentiation, showing promise for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Regenerative Medicine
Background:
- Xylan, a natural polysaccharide, is abundant and biocompatible.
- Developing in situ forming hydrogels is crucial for minimally invasive cell delivery.
- Bioconjugation strategies are needed to impart gelation properties to xylan.
Purpose of the Study:
- To synthesize an in situ forming hydrogel from spruce xylan.
- To evaluate the hydrogel's properties for cell encapsulation and in vivo delivery.
- To assess the viability and differentiation capacity of encapsulated cells.
Main Methods:
- Xylan was modified by reacting glucuronic acid groups with tyramine (TA).
- Enzymatic crosslinking of the TA-xylan conjugate induced rapid hydrogel formation.
- Mesenchymal stem cells were encapsulated, and their adipogenic differentiation was assessed.
Main Results:
- A 3D spruce xylan-based hydrogel formed rapidly (approx. 20s) via enzymatic crosslinking.
- The hydrogel demonstrated extensive swelling and maintained mechanical integrity for over two months.
- Encapsulated mesenchymal stem cells retained their adipogenic differentiation potential.
Conclusions:
- Spruce xylan is a viable precursor for developing in situ forming hydrogels.
- The synthesized hydrogel supports cell viability and function.
- This xylan-based hydrogel shows significant potential for tissue engineering and cell therapy.
