Novel sensory surface for creatine kinase electrochemical detection

Area of Science:

• Electrochemistry
• Biosensor Technology
• Biomarker Detection

Background:

• Creatine kinase isoenzyme (CK-MB) is a critical cardiac biomarker for ischemic conditions.
• Existing methods for CK-MB quantification can be complex and time-consuming.
• There is a need for rapid, sensitive, and selective point-of-care diagnostic tools.

Purpose of the Study:

• To develop a novel biosensor for quantifying CK-MB using immobilized phosphorylated creatine.
• To investigate the electrochemical properties and analytical performance of the developed biosensor.
• To evaluate the biosensor's applicability in biological fluids for point-of-care testing.

Main Methods:

• Immobilization of phosphorylated creatine onto aminated screen-printed electrodes via self-assembled monolayers.
• Electrochemical characterization using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV).
• Quantification of CK-MB using Square Wave Voltammetry (SWV) in mediator-free conditions.

Main Results:

• The biosensor demonstrated a limit of detection of 0.11 µg/mL for CK-MB.
• High selectivity was observed against potential interfering substances like troponin T, BSA, and myoglobin.
• The biosensor showed good stability at room temperature and successful application in biological fluids.

Conclusions:

• A novel, mediator-free biosensor for CK-MB detection based on immobilized phosphorylated creatine has been successfully developed.
• The biosensor exhibits excellent sensitivity, selectivity, and stability, making it suitable for clinical applications.
• This technology holds significant promise for point-of-care diagnostic applications due to its low cost and small device size.