Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

23.1K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.1K
Telomeres and Telomerase02:41

Telomeres and Telomerase

6.4K
6.4K
Replication in Eukaryotes01:29

Replication in Eukaryotes

15.4K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
15.4K
Replication in Eukaryotes02:31

Replication in Eukaryotes

156.9K
Overview
156.9K
Replicative Cell Senescence02:15

Replicative Cell Senescence

3.5K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.5K
Chromosome Replication02:31

Chromosome Replication

8.9K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conformational cycling of the Wntless transporter drives trafficking and secretion of Wnt morphogens.

Nature communications·2026
Same author

Structures of Cas9-Bound Double-Stranded DNA Mini-Circle Reveal Impacts of DNA Shape on Cas9 Target Interrogation.

bioRxiv : the preprint server for biology·2026
Same author

Cryo-EM Structure of a 95-Basepair Double-Stranded DNA Minicircle at 5.3 Å Resolution.

bioRxiv : the preprint server for biology·2026
Same author

Structures of human telomerase with BIBR1532 reveal novel mechanism of inhibition.

Nature chemical biology·2026
Same author

Cryo-EM structures reveal a conserved architecture for raiA noncoding RNA.

Nucleic acids research·2026
Same author

HEXIM1 inter-monomer autoinhibition governs 7SK RNA binding specificity and P-TEFb inactivation.

Nature communications·2026

Related Experiment Video

Updated: May 3, 2026

In vitro Reconstitution of the Active T. castaneum Telomerase
09:25

In vitro Reconstitution of the Active T. castaneum Telomerase

Published on: July 14, 2011

11.1K

Progress in structural studies of telomerase.

Edward J Miracco1, Jiansen Jiang2, Darian D Cash1

  • 1Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA.

Current Opinion in Structural Biology
|February 11, 2014
PubMed
Summary
This summary is machine-generated.

Recent advances reveal the structure of telomerase (ribonucleoprotein reverse transcriptase), crucial for chromosome maintenance, aging, and cancer. New electron microscopy data illuminate the organization of its protein and RNA components.

More Related Videos

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

11.7K
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

13.9K

Related Experiment Videos

Last Updated: May 3, 2026

In vitro Reconstitution of the Active T. castaneum Telomerase
09:25

In vitro Reconstitution of the Active T. castaneum Telomerase

Published on: July 14, 2011

11.1K
Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
08:26

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

Published on: June 12, 2018

11.7K
Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay
10:14

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

13.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Telomerase is a ribonucleoprotein (RNP) reverse transcriptase essential for maintaining the 3' ends of linear chromosomes.
  • It plays significant roles in cellular aging, stem cell renewal, and tumorigenesis.
  • Understanding telomerase structure is key to elucidating its function and therapeutic targeting.

Purpose of the Study:

  • To present recent breakthroughs in determining the architecture of the telomerase holoenzyme.
  • To describe the structural basis of telomerase activity.
  • To provide insights into the organization of proteins and RNA within the telomerase RNP complex.

Main Methods:

  • Electron microscopy (EM) was utilized to determine the structures of the Tetrahymena thermophila telomerase holoenzyme and a human telomerase dimer.
  • Structural analysis focused on telomerase reverse transcriptase (TERT) and telomerase RNA (TER) domains.
  • Structures of telomerase protein domains beyond TERT, and their complexes with TER or telomeric single-stranded DNA, were determined.

Main Results:

  • The first electron microscopy structures of the telomerase holoenzyme and a human telomerase dimer were reported.
  • New structures revealed details of TERT and TER domains, as well as telomerase protein domains beyond TERT.
  • Complexes of these domains with TER or telomeric single-stranded DNA were structurally characterized.

Conclusions:

  • These structural studies offer the first comprehensive view of the organization of proteins and RNA within the telomerase RNP.
  • The findings advance our understanding of the molecular mechanisms underlying telomerase function.
  • This structural information is foundational for future research into telomerase-related diseases and therapeutic development.