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Cryo-electron Microscopy

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Updated: Jun 5, 2026

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Cryo-EM Structure of a 95-Basepair Double-Stranded DNA Minicircle at 5.3 Å Resolution.

Yukang Liu, Kyu-Yeon Lee, Yao He

    Biorxiv : the Preprint Server for Biology
    |June 4, 2026
    PubMed
    Summary

    Researchers determined the cryo-EM structure of a 95-basepair double-stranded DNA minicircle (dsMC95). This high-resolution structure reveals a stable B-DNA configuration, offering insights into DNA under topological constraints.

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    Area of Science:

    • Structural Biology
    • Biophysics
    • Molecular Biology

    Background:

    • Double-stranded DNA minicircles are prevalent in biological systems.
    • They are extensively utilized in biotechnology, therapeutics, and research.

    Purpose of the Study:

    • To determine the high-resolution cryo-electron microscopy (cryo-EM) structure of a 95-basepair double-stranded DNA minicircle (dsMC95).
    • To analyze the structural features and conformation of dsMC95 under topological constraints.

    Main Methods:

    • Cryo-electron microscopy (cryo-EM) was employed to obtain the structure of dsMC95.
    • High-resolution structural analysis was performed at 5.3 Å.

    Main Results:

    • The structure of dsMC95 was resolved at 5.3 Å, showing a closed ring with no local deformation.
    • Analysis confirmed a B-DNA configuration with a helical twist periodicity of nine-fold (10.56 bp/turn).
    • The minicircle exhibited in-plane ellipticity and out-of-plane displacement, with variations between segments.

    Conclusions:

    • The high-resolution dsMC95 structure provides a detailed view of DNA under topological constraint.
    • This work advances understanding of DNA physical properties like bending and informs nanotechnology applications.