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Updated: May 3, 2026
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
Preparation and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids
Ali Mirsaidi1, André N Tiaden1, Peter J Richards1
1Bone and Stem Cell Research Group, CABMM, University of Zurich, Zurich, Switzerland.
Abstract:
In this unit, previously described methods are expanded upon, where procedures relating to the preparation, culturing, and osteogenic differentiation of scaffold-free mouse adipose-derived stromal cell microtissue spheroids (ASC-MT) are outlined. Not only is a detailed methodology of how to engineer such spheroids are presented, but a full account of how to induce and analyze osteogenesis in these ASC-MT constructs is given along with relevant figures to help better illustrate the methods described.
