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Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
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Quantitative analysis of copy number variants based on real-time LightCycler PCR
Lijiang Ma1, Wendy K Chung1,2
1Department of Pediatrics, Columbia University, New York, New York.
Current Protocols in Human Genetics
|February 11, 2014
Abstract:
Quantitative real-time PCR is PCR visualized in real time by the use of fluorescent or intercalating dyes, which are employed to measure gene expression or gene quantification including contiguous gene deletions or duplications. A simple method is described here to quantify DNA copy number from human samples.
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