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Purification of fibronectin
1National Institute of Environmental Health Sciences, NIH, Research Triangle Park, North Carolina.
Current Protocols in Cell Biology
|February 11, 2014
Summary
This study details fibronectin purification from plasma and cells using affinity chromatography. High-purity fibronectin is readily obtained for cell adhesion and migration research.
Area of Science:
- Biochemistry
- Cell Biology
- Proteomics
Background:
- Fibronectin is a crucial multifunctional adhesive glycoprotein involved in cell adhesion and migration.
- Purification of fibronectin is essential for its use in various biological assays.
- Existing methods may be complex or yield lower purity fibronectin.
Purpose of the Study:
- To describe a straightforward method for purifying fibronectin.
- To enable the isolation of both plasma-derived and cell-derived fibronectin.
- To provide high-purity fibronectin for cell-based assays.
Main Methods:
- Affinity chromatography is employed for fibronectin purification.
- The protocol covers purification from both plasma and cell sources (cell surfaces and conditioned medium).
- Simple techniques are utilized to ensure accessibility.
Main Results:
- High purity levels of fibronectin are achievable.
- The purification process is efficient and relatively simple.
- Obtained fibronectin is suitable for downstream applications.
Conclusions:
- Simple affinity chromatography is an effective method for obtaining high-purity fibronectin.
- This protocol facilitates the use of fibronectin in cell adhesion and migration studies.
- The described purification strategy is valuable for researchers in cell biology and biochemistry.
