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Related Experiment Video

Updated: May 3, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

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Superresolution imaging with standard fluorescent probes.

Bryan A Millis1, Dylan T Burnette2, Jennifer Lippincott-Schwartz2

  • 1Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, Maryland.

Current Protocols in Cell Biology
|February 11, 2014
PubMed
Summary
This summary is machine-generated.

Superresolution microscopy overcomes light

Keywords:
BaLMbleachingblinkingmicroscopysuperresolution

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Area of Science:

  • Microscopy
  • Biophysics
  • Optical Engineering

Background:

  • Diffraction limits optical microscopy resolution to ~200 nm.
  • Superresolution microscopy combines advanced optics, illumination, and computation.
  • Existing superresolution techniques have limitations in probe and condition suitability.

Purpose of the Study:

  • To introduce a new superresolution technique called bleaching/blinking-assisted localization microscopy (BaLM).
  • To leverage inherent fluorophore properties for enhanced imaging resolution.
  • To overcome limitations of current superresolution methods.

Main Methods:

  • Developed and applied bleaching/blinking-assisted localization microscopy (BaLM).
  • Utilized intrinsic blinking and bleaching characteristics of fluorophores.
  • Implemented computational analysis for image reconstruction.

Main Results:

  • Demonstrated superresolution imaging capabilities using BaLM.
  • Successfully generated superresolution images by exploiting fluorophore blinking/bleaching.
  • Showcased a novel approach to superresolution microscopy.

Conclusions:

  • BaLM offers a new method for achieving superresolution.
  • The technique utilizes common fluorophore properties, expanding imaging possibilities.
  • This approach addresses limitations in probe and condition compatibility for superresolution imaging.