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Updated: Jul 28, 2026
In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
Published on: March 19, 2010
A cell-free approach to accelerate the study of protein-protein interactions in vitro
E Sierecki1, N Giles1, M Polinkovsky1
1Institute for Molecular Bioscience , University of Queensland , Saint Lucia, Queensland , Australia.
Abstract:
Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
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02:04Protein-protein Interfaces
02:26Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...