Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Absorption of Nutrients01:19

Absorption of Nutrients

14.5K
Absorption refers to taking dietary nutrients from the intestinal lumen for transportation throughout the body. After digestion in the small intestine, carbohydrates, proteins, and fats are broken down into simpler forms. These essential macronutrients and other vital substances, such as vitamins, minerals, and water, are then prepared for absorption into the bloodstream.
Enterocytes, which are specialized polar epithelial cells, line the mucosa of the small intestinal walls. These cells...
14.5K
Factors Influencing Drug Absorption: Anatomical Parameters01:23

Factors Influencing Drug Absorption: Anatomical Parameters

930
Drug absorption involves the movement of drugs from the point of administration into the systemic circulation. Initially, Gastrointestinal (GI) motility propels the drug through the digestive tract and into the stomach. However, the stomach's high acidity and limited surface area restrict its role in drug absorption for most drugs. The drug then moves from the stomach to the small intestine via gastric emptying, which can be slowed by various factors, including interactions with other...
930
Drug Absorption: Factors Affecting GI Absorption01:19

Drug Absorption: Factors Affecting GI Absorption

6.2K
The process of oral drug absorption can be influenced by several factors. Weakly acidic drugs tend to be absorbed more readily from the stomach due to their nonionized state. However, absorption may be less efficient in the upper intestine, where drugs are often ionized. Interestingly, despite the stomach's apparent advantage for drug absorption, its mucous layer can hinder diffusion. Its surface area is also smaller than the intestine's, which can further slow down the absorption rate.
6.2K
Mineral, Vitamin and Water Absorption01:27

Mineral, Vitamin and Water Absorption

2.2K
Electrolytes are essential minerals and ions primarily obtained from the diet and absorbed through the gastrointestinal tract. Most electrolytes are absorbed in the small intestine. While the absorption of iron and calcium primarily occurs in the duodenum, calcium is also absorbed in the jejunum and ileum. In these regions, passive diffusion contributes to its absorption alongside active transport mechanisms in the duodenum. These ions can exit the enterocytes through specialized active...
2.2K
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

887
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
887
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

844
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
844

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biochemistry at scale: Seeing both the forest and the trees.

Trends in biochemical sciences·2026
Same author

Intersection of Sphingolipid and Sterol Metabolism at the Level of Orm Proteins in Yeast.

Cells·2026
Same author

Chromatin rewiring of β-globin and MYC enhancers by TGF-β1 drives defective erythropoiesis.

Nature communications·2026
Same author

Diagnosing idiosyncratic drug-induced liver injury: lessons from monocyte-derived hepatocyte-like cells.

Expert opinion on drug metabolism & toxicology·2026
Same author

Pyrimethamine overcomes resistance to hypomethylating agents by reducing de novo pyrimidine synthesis.

Experimental hematology·2026
Same author

Cerebral Folate Deficiency, Autism, and the Role of Leucovorin.

The New England journal of medicine·2026

Related Experiment Video

Updated: May 3, 2026

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
08:42

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport

Published on: November 27, 2016

10.5K

The intestinal absorption of folates.

Michele Visentin1, Ndeye Diop-Bove, Rongbao Zhao

  • 1Departments of Molecular Pharmacology and Medicine, Albert Einstein College of Medicine, Bronx, New York 10461; email: michele.visentin@einstein.yu.edu , ndeye.diop-bove@einstein.yu.edu , rongbao.zhao@einstein.yu.edu , i.david.goldman@einstein.yu.edu.

Annual Review of Physiology
|February 12, 2014
PubMed
Summary

The proton-coupled folate transporter (PCFT) is crucial for intestinal folate absorption. This review details PCFT

More Related Videos

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

1.6K
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

7.5K

Related Experiment Videos

Last Updated: May 3, 2026

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
08:42

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport

Published on: November 27, 2016

10.5K
Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

1.6K
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

7.5K

Area of Science:

  • Gastroenterology and Nutrition Science
  • Molecular Biology
  • Human Physiology

Background:

  • Intestinal folate absorption mechanisms were historically unclear.
  • The proton-coupled folate transporter (PCFT) was recently identified.
  • PCFT's role was confirmed by hereditary folate malabsorption and Pcft-null mouse models.

Purpose of the Study:

  • To review the properties of PCFT-mediated folate transport.
  • To compare PCFT transport with the reduced folate carrier.
  • To discuss other folate transport processes and unresolved issues.

Main Methods:

  • Review of existing literature on PCFT and folate transport.
  • Analysis of genetic data from hereditary folate malabsorption.
  • Examination of data from Pcft-null mouse models.

Main Results:

  • PCFT is critical for folate absorption in the proximal small intestine.
  • PCFT-mediated transport differs from reduced folate carrier transport.
  • Factors influencing PCFT activity, including ions, molecules, and drugs, are described.

Conclusions:

  • PCFT is the primary transporter for intestinal folate absorption.
  • Further research is needed on PCFT gene regulation and absorption mechanisms without PCFT.
  • Understanding PCFT is vital for addressing folate deficiencies and related disorders.