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Updated: May 3, 2026

Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
Published on: April 10, 2016
Tomographic diffractive microscopy and multiview profilometry with flexible aberration correction
Abstract:
We have developed a tomographic diffractive microscope in reflection, which permits observation of sample surfaces with an improved lateral resolution, compared to a conventional holographic microscope. From the same set of data, high-precision measurements can be performed on the shape of the reflective surface by reconstructing the phase of the diffracted field. Doing so allows for several advantages compared to classical holographic interferometric measurements: improvement in lateral resolution, easier phase unwrapping, reduction of the coherent noise, combined with the high-longitudinal precision provided by interferometric phase measurements. We demonstrate these capabilities by imaging various test samples.
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