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Iron pyronine and alcian blue for staining acid mucin in plastic-embedded sections
M S Cannon1, N R Stuth, J J Darcy
1Department of Anatomy, College of Medicine, Texas A & M University, College Station 77843.
American Journal of Clinical Pathology
|April 1, 1988
Abstract:
Iron pyronine Y and Alcian blue demonstrated sulfated and nonsulfated acid mucin, respectively, in plastic-embedded sections. Safran used in combination with these two dyes stained collagen and some reticulum fibers. Sulfated acid mucin was red, while nonsulfated acid mucin stained blue; collagen appeared yellow to greenish yellow. The iron pyronine Y-Alcian blue-safran staining method, when used as in the present article, produces excellent cellular visualization of sulfated and nonsulfated acid mucin.