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Updated: May 3, 2026
10:32
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Published on: June 5, 2015
18.3K
Human B cell immortalization for monoclonal antibody production
Joyce Hui-Yuen1, Siva Koganti, Sumita Bhaduri-McIntosh
1Pediatric Infectious Diseases, Department of Pediatrics, Stony Brook University School of Medicine, Stony Brook, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2014
Summary
This study presents a streamlined method for generating Epstein-Barr virus-transformed lymphoblastoid cell lines (LCL) from peripheral blood. The technique ensures rapid immortalization and cryopreservation for efficient use in immunological assays.
Area of Science:
- Immunology
- Virology
- Cell Biology
Background:
- Primary B lymphocytes are crucial for adaptive immunity.
- Epstein-Barr virus (EBV) infection induces B cell immortalization.
- Lymphoblastoid cell lines (LCL) are valuable tools in immunological research.
Purpose of the Study:
- To describe an optimized method for generating lymphoblastoid cell lines (LCL).
- To achieve rapid immortalization and cryopreservation of LCL.
- To facilitate the use of LCL in immunological assays and antibody generation.
Main Methods:
- Infection of primary B lymphocytes with Epstein-Barr virus.
- Culturing and immortalization of B cells.
- Cryopreservation of generated LCL.
Main Results:
- Successful generation of growth-transformed and immortalized LCL.
- Demonstration of rapid immortalization and cryopreservation.
- Validation of LCL for antigen presentation and antibody production.
Conclusions:
- The described method provides an efficient approach for LCL generation.
- Rapid immortalization and cryopreservation enhance LCL utility.
- Generated LCL serve as critical reagents in immunological studies.
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