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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid

Nicole Müller-Sienerth1, Cécile Crosnier, Gavin J Wright

  • 1Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2014
PubMed

Antibodies are an integral part of biological and medical research. In addition, immunoglobulins are used in many diagnostic tests and are becoming increasingly important in the therapy of diseases. To express antibodies recombinantly, the immunoglobulin heavy and light chains are usually cloned into two different expression plasmids. Here, we describe a method for recombinant antibody expression from a single plasmid.

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