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Updated: Feb 6, 2026

Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
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Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification

Emil Bujak1, Mattia Matasci, Dario Neri

  • 1Philochem AG, Zurich, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2014
PubMed

The scFv-Fc format allows for rapid characterization of candidate scFvs isolated from phage display libraries before conversion into a full-length IgG. This format offers several advantages over the phage display-derived scFv, including bivalent binding, longer half-life, and Fc-mediated effector functions. Here, a detailed method is presented, which describes the cloning, expression, and purification of an scFv-Fc fragment, starting from scFv fragments obtained from a phage display library. This method facilitates the rapid screening of candidate antibodies, prior to a more time-consuming conversion into a full IgG format. Alternatively, the scFv-Fc format may be used in the clinic for therapeutic applications.

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