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Updated: May 3, 2026
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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
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Antibody V and C domain sequence, structure, and interaction analysis with special reference to IMGT®
Eltaf Alamyar1, Véronique Giudicelli, Patrice Duroux
1The International ImMunoGenetics information system, Laboratoire d'ImmunoGénétique Moléculaire, Institut de Génétique Humaine IGH, Université Montpellier 2, Montpellier, France.
Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2014
Summary
The International ImMunoGeneTics information system (IMGT) provides standardized immunogenetics data and tools. IMGT ensures accuracy for immunoglobulin and T cell receptor analysis, aiding antibody engineering.
Area of Science:
- Immunogenetics
- Immunoinformatics
- Structural Biology
Background:
- IMGT is the global reference for immunogenetics and immunoinformatics.
- IMGT ensures data accuracy and consistency through IMGT-ONTOLOGY, linking genes, sequences, and 3D structures.
- IMGT applies standardized rules and nomenclature across different molecule types and species.
Purpose of the Study:
- To present the IMGT tools and databases for standardized analysis of immunoglobulin (IG) and T cell receptor (TR) V and C domains.
- To highlight the capabilities of IMGT/V-QUEST, IMGT/HighV-QUEST, IMGT/DomainGapAlign, and IMGT/3Dstructure-DB.
- To emphasize the utility of IMGT resources for antibody engineering and humanization.
Main Methods:
- IMGT/V-QUEST for V domain analysis of IG/TR rearranged sequences, integrating IMGT/JunctionAnalysis and IMGT/Automat.
- IMGT/HighV-QUEST for high-throughput sequencing data analysis with statistical capabilities.
- IMGT/DomainGapAlign for V and C domain amino acid sequence analysis and IMGT/3Dstructure-DB for 3D structure and interaction analysis.
Main Results:
- IMGT provides highly standardized analysis of IG and TR V and C domains.
- IMGT/HighV-QUEST enables high-throughput analysis of up to 150,000 sequences per batch.
- IMGT/3Dstructure-DB and associated tools offer insights into 3D structures and paratope/epitope interactions.
Conclusions:
- IMGT tools and databases are invaluable for antibody engineering and humanization.
- IMGT's standardized approach ensures high-quality data and analysis.
- IMGT/mAb-DB provides access to therapeutic antibody data, further supporting antibody development.
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