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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 3, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Antibody array generation and use.

Carl A K Borrebaeck¹, Christer Wingren

¹Department of Immunotechnology and CREATE Health, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)

|February 12, 2014

Summary

Antibody arrays enable high-throughput protein expression profiling for biomarker discovery and disease diagnostics. This multiplex technology offers sensitive detection and quantification of proteins in serum samples.

Area of Science:

Biochemistry and Molecular Biology
Immunology
Proteomics

Background:

Affinity proteomics utilizes antibody arrays for multiplexed, high-throughput protein expression profiling.
This technology allows for specific, sensitive, and miniaturized analysis of crude proteomes.
Antibody arrays involve depositing antibodies onto a solid support for subsequent protein binding and detection.

Purpose of the Study:

To describe the generation and application of planar antibody arrays for serum protein profiling.
To highlight the utility of antibody arrays in protein expression analysis.
To showcase the potential of this technology in various diagnostic and prognostic applications.

Main Methods:

Generation of planar antibody arrays by ordered deposition of antibodies onto a solid support.

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Incubation of arrays with biological samples (e.g., serum) to allow specific protein binding.

Detection and quantification of bound proteins, primarily using fluorescence-based methods.

Conversion of binding patterns into relative protein expression maps (protein atlases).

Main Results:

Demonstration of antibody arrays as a viable method for serum protein profiling.
Generation of relative protein expression maps delineating molecular composition.
Successful application of the technology for high-throughput analysis.

Conclusions:

Planar antibody arrays are effective for sensitive and specific protein expression profiling.
This technology supports applications in biomarker discovery, disease diagnostics, and monitoring.
Antibody arrays provide a powerful tool for molecular-level characterization of biological samples.