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Updated: May 3, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Published on: February 23, 2018
Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
C A G Leal1, G A Carvalho-Castro1, A C Cottorello2
1Laboratório de Doenças de Animais Aquáticos, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Abstract:
The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
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