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Updated: May 3, 2026

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
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Age-dependent changes in stratum corneum barrier function.

E Boireau-Adamezyk1, A Baillet-Guffroy, G N Stamatas

  • 1Faculté de pharmacie, EA 4041, Université Paris Sud 11, Châtenay-Malabry Cedex, France.

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [And] International Society for Digital Imaging of Skin (ISDIS) [And] International Society for Skin Imaging (ISSI)
|February 13, 2014
PubMed
Summary
This summary is machine-generated.

Skin aging affects the stratum corneum (SC) barrier function, with SC thickening with age compensating for reduced lipid organization. These changes vary by body site and environmental exposure.

Keywords:
barrier functionraman spectroscopyskin agingskin lipids

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Area of Science:

  • Dermatology
  • Skin Physiology
  • Aging Research

Background:

  • The stratum corneum (SC) is crucial for skin barrier function, influenced by its structure, composition, and lipid organization.
  • Understanding age-dependent changes in SC barrier function is vital for dermatological research.

Purpose of the Study:

  • To evaluate age-related alterations in SC barrier function.
  • To investigate associated physiological changes and their correlation with aging.

Main Methods:

  • Studied 40 French women across four age groups.
  • Analyzed SC composition (water, lipids, proteins, cholesterol, ceramides) using Raman confocal microspectroscopy.
  • Measured skin hydration via skin conductance and barrier function via transepidermal water loss (TEWL).

Main Results:

  • Transepidermal water loss (TEWL) slightly decreases with age, linked to increased SC thickness, more rapidly on the face.
  • Lipid-to-protein ratio and lipid compactness decline with age, particularly on arm sites.
  • Cheek SC exhibited higher TEWL, water, and lipid content, with less thickness than arm sites across all ages.

Conclusions:

  • Skin aging, body site, and environmental exposure impact SC barrier function, structure, and lipid content.
  • Increased SC thickness with age compensates for reduced lipid quantity and organization.
  • These findings highlight the complex interplay of factors affecting skin barrier integrity over time.